https://www.sevivo.com/about daily 0.75 2020-10-19 https://static1.squarespace.com/static/5927959437c58161a8f3fcc8/t/5a9878f7652deaafd1f3a4ed/1519941880175/met_trans.jpg Contact https://www.sevivo.com/assay-tools daily 0.75 2020-10-19 https://static1.squarespace.com/static/5927959437c58161a8f3fcc8/t/5f8ddeb8ca67d16034da76b9/1603133134208/MTase.gif Assay Tools https://static1.squarespace.com/static/5927959437c58161a8f3fcc8/t/5f8ddedc8b51110304f93eb8/1603133162894/Deadenylase.gif Assay Tools https://static1.squarespace.com/static/5927959437c58161a8f3fcc8/t/5f8ddefa6afd8704c6a47ff6/1603133190161/Radical-SAM.gif Assay Tools https://www.sevivo.com/home daily 1.0 2020-10-19 https://static1.squarespace.com/static/5927959437c58161a8f3fcc8/t/5f8deda4173f556934572512/1603137096596/Banner.jpg Home https://www.sevivo.com/news daily 0.75 2020-10-20 https://www.sevivo.com/cloning-tools daily 0.75 2020-11-10 https://static1.squarespace.com/static/5927959437c58161a8f3fcc8/t/5f8cdb4bbeb5016c3614b59e/1603167328270/cloning.jpg Cloning Tools Parallelize optimization of fusion protein production to accelerate testing for maximizing protein yield. Plasmids encode six different N-terminal fusions and a 3C Protease cleavage site. Using a PCR amplicon made with appropriate primers, the linearized plasmids, and your gene assembly kit of choice, you can incorporate the gene-of-interest into all six plasmids in a single recombination reaction and transformation. https://static1.squarespace.com/static/5927959437c58161a8f3fcc8/t/5faaffa7f2259501089503c7/1605042097706/Plasmid%252BCharacterization.jpg Cloning Tools The gene inserted into any plasmid can be sequenced using a Universal primer compatible will all plasmids. Combined with Promoter and Terminator primers, one can fully characterize and validate their expression plasmid. https://static1.squarespace.com/static/5927959437c58161a8f3fcc8/t/5faaffe99d4b8b7ae361131f/1605042164058/R3CP%2BCleavage.jpg Cloning Tools The recombinant tagged protein of interest, following purification with IMAC, is cleaved by the His-tagged RP at the RP Cut Site (RP-CS). Both the His-tagged fusion partner and His-tagged protease are then removed using a second IMAC step. This typically results in highly pure Protein of Interest.